Hotspot

rpl14-rpl16

LSC · 80298-80768 · 471 bp

Back to Lygodium

Pi 0.1

MarkerSeek score 80.70

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 121
pi: 0.1
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TTCGGTTACAACACTTCGGG	GTATGGGATCCGGTAAGGGA	50.0	55.0	58.1	58.0	982-1015 bp	1.0	90.8
rpl14-rpl16_p2	TCCGTTTCTGCGCTTTAACT	GTATGGGATCCGGTAAGGGA	45.0	55.0	58.1	58.0	833-863 bp	0.875	85.3
rpl14-rpl16_p3	TCCGTTTCTGCGCTTTAACT	GCGAAATAGGAACCACGAGT	45.0	50.0	58.1	58.0	1162-1192 bp	0.875	84.5
rpl14-rpl16_p4	TTCGGTTACAACACTTCGGG	GCGAAATAGGAACCACGAGT	50.0	50.0	58.1	58.0	1314-1344 bp	0.875	84.1
rpl14-rpl16_p5	TGTCGGCTACGTTTAAGCAA	GCGAAATAGGAACCACGAGT	45.0	50.0	57.8	58.0	1007 bp	0.125	43.7