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psbE-petL

LSC · 63400-64650 · 1251 bp

Back to Lygodium

Pi 0.0625

MarkerSeek score 85.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1251
variable_sites: 249
pi: 0.0625
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CGCGATGAACAGTGAAGGTA	AGCGCCTTTAGTTCAGTCTG	50.0	50.0	58.0	57.9	1985-2052 bp	1.0	89.5
psbE-petL_p2	CGAAGACGTCATAGGCCAAA	AGCGCCTTTAGTTCAGTCTG	50.0	50.0	58.0	57.9	2028-2095 bp	0.875	83.2
psbE-petL_p3	CGCGATGAACAGTGAAGGTA	ATACGTCAAGAAGCGCCTTT	50.0	45.0	58.0	57.9	1996-2063 bp	0.625	70.8
psbE-petL_p4	CGAAGACGTCATAGGCCAAA	ATACGTCAAGAAGCGCCTTT	50.0	45.0	58.0	57.9	2039-2106 bp	0.625	70.7
psbE-petL_p5	AGGAGTCCCCAATAAAGGCT	AGCGCCTTTAGTTCAGTCTG	50.0	50.0	58.0	57.9	2361-2422 bp	0.5	69.0