Hotspot

accD-psaI

LSC · 57506-57971 · 466 bp

Back to Lygodium

Pi 0.0547

MarkerSeek score 82.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 466
variable_sites: 79
pi: 0.0547
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCACAGGTTCGCAGGAAAAA	TGTGCGAGAACCTTTGACAA	45.0	45.0	58.0	58.0	1575-1599 bp	1.0	88.6
accD-psaI_p2	TACGGAAAGGACTGAACCCT	TGTGCGAGAACCTTTGACAA	50.0	45.0	58.0	58.0	1688-1706 bp	0.75	78.7
accD-psaI_p3	TACGGAAAGGACTGAACCCT	TTCGGACGAAAGCAAGGAAA	50.0	45.0	58.0	58.0	1799-1817 bp	0.625	72.8
accD-psaI_p4	TACGGAAAGGACTGAACCCT	ACCGAACCACCACAAATTGA	50.0	45.0	58.0	57.9	1327-1338 bp	0.625	70.6
accD-psaI_p5	TACGGAAAGGACTGAACCCT	AGAAGGACAAGGAGCAGCTA	50.0	50.0	58.0	58.1	1458-1469 bp	0.5	65.2