Hotspot

ndhC-trnV-UAC

LSC · 50398-50940 · 543 bp

Back to Lygodium

Pi 0.0652

MarkerSeek score 84.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 543
variable_sites: 109
pi: 0.0652
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GAAACCGGATCCAGATGTCC	GACGTGTCGATATGAGAGCC	55.0	55.0	58.0	58.0	842-884 bp	1.0	91.3
ndhC-trnV-UAC_p2	TCAATGAAGCAACAGCTGGT	GACGTGTCGATATGAGAGCC	45.0	55.0	58.0	58.0	1223-1265 bp	1.0	90.4
ndhC-trnV-UAC_p3	TCAATGAAGCAACAGCTGGT	GGTTCGAATCCGTATAGCCC	45.0	55.0	58.0	57.9	1092-1129 bp	1.0	90.1
ndhC-trnV-UAC_p4	TCACATATTTGGGCTCTGGC	GACGTGTCGATATGAGAGCC	50.0	55.0	57.9	58.0	1390-1432 bp	1.0	89.7
ndhC-trnV-UAC_p5	TCAATGAAGCAACAGCTGGT	CATGTTGGGTTCGTAGAGCA	45.0	50.0	58.0	57.9	1220 bp	0.125	44.1