Hotspot

rps4-trnL-CAA

LSC · 46844-47504 · 661 bp

Back to Lygodium

Pi 0.0562

MarkerSeek score 82.90

Primer available yes

Label rps4-trnL-CAA

Feature Metadata

label_name: rps4-trnL-CAA
feature_type: igs
region: LSC
length_bp: 661
variable_sites: 101
pi: 0.0562
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-CAA_p1	GCGTTCTGTTGATCCGTAGT	CTGAGAGCTACCACTCGGTA	50.0	55.0	58.0	58.0	904-938 bp	1.0	91.1
rps4-trnL-CAA_p2	GCGTTCTGTTGATCCGTAGT	TTTCTGGAGTGTTCACCTGC	50.0	50.0	58.0	58.0	1037-1071 bp	0.75	79.0
rps4-trnL-CAA_p3	GATACCTGACCCGTTGAACC	TTTCTGGAGTGTTCACCTGC	55.0	50.0	58.0	58.0	1096-1130 bp	0.75	78.4
rps4-trnL-CAA_p4	GCGTTCTGTTGATCCGTAGT	CCCTGGATCTGAGATTTGGC	50.0	55.0	58.0	58.0	967-997 bp	0.5	68.9
rps4-trnL-CAA_p5	GATACCTGACCCGTTGAACC	CCCTGGATCTGAGATTTGGC	55.0	55.0	58.0	58.0	1026-1056 bp	0.5	68.4