Hotspot

trnS-UGA-psbC

LSC · 32339-33184 · 846 bp

Back to Lygodium

Pi 0.0651

MarkerSeek score 84.40

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 152
pi: 0.0651
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GTTTGTACCATCCGATCCCC	CGATACCGAACCCGTTCTTT	55.0	50.0	58.0	58.0	1303-1338 bp	0.875	85.6
trnS-UGA-psbC_p2	TGGCGTATTCGTTCCTCAAG	CGATACCGAACCCGTTCTTT	50.0	50.0	58.0	58.0	1540-1575 bp	0.5	69.4
trnS-UGA-psbC_p3	ACGGATGAACATCGATGCAA	CGATACCGAACCCGTTCTTT	45.0	50.0	58.0	58.0	1061-1095 bp	0.5	69.1
trnS-UGA-psbC_p4	TGGCGTATTCGTTCCTCAAG	GTTTCTCCCCGAAGTTGGTT	50.0	50.0	58.0	58.0	1665-1700 bp	0.5	68.5
trnS-UGA-psbC_p5	TCCAGCACCCTAAGATCCAA	CGATACCGAACCCGTTCTTT	50.0	50.0	58.0	58.0	1244-1259 bp	0.375	61.8