Hotspot

trnE-UUC-trnG-GCC

LSC · 30560-31259 · 700 bp

Back to Lygodium

Pi 0.0721

MarkerSeek score 84.90

Primer available yes

Label trnE-UUC-trnG-GCC

Feature Metadata

label_name: trnE-UUC-trnG-GCC
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 151
pi: 0.0721
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnG-GCC_p1	CCTAGGCCTCTAGACGATGG	GCGCGGATATAGTTGAATGG	60.0	50.0	58.5	56.8	759-797 bp	1.0	79.0
trnE-UUC-trnG-GCC_p2	CTAGGCCTCTAGACGATGGG	GCGCGGATATAGTTGAATGG	60.0	50.0	58.5	56.8	758-796 bp	1.0	79.0
trnE-UUC-trnG-GCC_p3	CCTAGGCCTCTAGACGATGG	GTTCGATTCCCGCTATCCG	60.0	57.9	58.5	58.2	707-745 bp	1.0	78.8
trnE-UUC-trnG-GCC_p4	CTAGGCCTCTAGACGATGGG	GTTCGATTCCCGCTATCCG	60.0	57.9	58.5	58.2	706-744 bp	1.0	78.8
trnE-UUC-trnG-GCC_p5	CTAGGCCTCTAGACGATGGG	GGTTCGATTCCCGCTATCC	60.0	57.9	58.5	57.8	707-745 bp	1.0	78.6