Hotspot

rpoB-trnC-GCA

LSC · 26449-27320 · 872 bp

Back to Lygodium

Pi 0.0848

MarkerSeek score 83.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 199
pi: 0.0848
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GGGACTCCTCAGTACTTGGT	AACCTTGGATTAGGCGACAC	55.0	50.0	58.1	57.9	1287-1321 bp	0.875	85.1
rpoB-trnC-GCA_p2	GGGACTCCTCAGTACTTGGT	ATGGATGGTCAGTCGGAGAT	55.0	50.0	58.1	57.9	1615-1649 bp	0.5	69.6
rpoB-trnC-GCA_p3	TATGGTCTGATTCGCGGTTG	ATGGATGGTCAGTCGGAGAT	50.0	50.0	58.1	57.9	1646-1680 bp	0.5	69.5
rpoB-trnC-GCA_p4	GATAAGGACCCTCGCGATTC	ATGGATGGTCAGTCGGAGAT	55.0	50.0	57.9	57.9	1594-1628 bp	0.5	69.5
rpoB-trnC-GCA_p5	TGCATTGGGGTACAAATGCT	ATGGATGGTCAGTCGGAGAT	45.0	50.0	58.1	57.9	1924-1958 bp	0.5	69.1