Hotspot

atpH-atpI

LSC · 14319-14997 · 679 bp

Back to Lygodium

Pi 0.0742

MarkerSeek score 83.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 151
pi: 0.0742
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TGTCTCGCAATGCCTTCTAC	ACCAGGACTCAAATGGGAGA	50.0	50.0	58.0	58.0	1286-1316 bp	1.0	91.3
atpH-atpI_p2	TGTCTCGCAATGCCTTCTAC	ACATCCAACCGACTCCAGTA	50.0	50.0	58.0	58.1	1039-1069 bp	0.875	84.9
atpH-atpI_p3	TGTCTCGCAATGCCTTCTAC	AGTACATCCAACCGACTCCA	50.0	50.0	58.0	58.1	1042-1072 bp	0.875	84.9
atpH-atpI_p4	TGTCTCGCAATGCCTTCTAC	TACATCCAACCGACTCCAGT	50.0	50.0	58.0	58.1	1040-1070 bp	0.875	84.9
atpH-atpI_p5	GTTTATGGTCTCGTCCCCAC	ACCAGGACTCAAATGGGAGA	55.0	50.0	58.0	58.0	1613-1639 bp	0.625	75.1