Hotspot

ycf1

SSC · 127201-132651 · 5451 bp

Back to Lycoris

Pi 0.00498

MarkerSeek score 34.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5451
variable_sites: 96
pi: 0.00498
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTATTTTGGCGACCCACCTT	GTCTGAAACCTTGGCGAAGA	50.0	50.0	58.2	58.1	294 bp	1.0	79.9
ycf1_p2	CTATTTTGGCGACCCACCTT	CTGTCTGAAACCTTGGCGAA	50.0	50.0	58.2	58.4	296 bp	1.0	77.6
ycf1_p3	CTATTTTGGCGACCCACCTT	TGTCTGAAACCTTGGCGAAG	50.0	50.0	58.2	58.4	295 bp	1.0	77.6
ycf1_p4	CTATTTTGGCGACCCACCTT	TTCTGTCTGAAACCTTGGCG	50.0	50.0	58.2	58.4	298 bp	1.0	77.6
ycf1_p5	TATTTTGGCGACCCACCTTG	GTCTGAAACCTTGGCGAAGA	50.0	50.0	58.5	58.1	293 bp	1.0	77.5