Hotspot

ndhD

SSC · 118691-120196 · 1506 bp

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Pi 0.00526

MarkerSeek score 38.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 26
pi: 0.00526
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGTGTTGTACAGCGAATGA	TGGCCAAAACGTATCTTGTCT	40.0	42.9	55.6	57.9	1618 bp	1.0	53.4
ndhD_p2	TTTGTGTTGTACAGCGAATGA	TGGCCAAAACGTATCTTGTCT	38.1	42.9	56.4	57.9	1619 bp	1.0	51.6
ndhD_p3	TGTGTTGTACAGCGAATGAT	TGGCCAAAACGTATCTTGTCT	40.0	42.9	55.4	57.9	1617 bp	1.0	51.5
ndhD_p4	TTGTGTTGTACAGCGAATGA	CACGGGTTTTTGGCCAAAA	40.0	47.4	55.6	57.5	1628 bp	1.0	50.7
ndhD_p5	TGTGTTGTACAGCGAATGATT	TGGCCAAAACGTATCTTGTCT	38.1	42.9	56.2	57.9	1617 bp	1.0	49.8