Hotspot

atpF

LSC · 12495-13974 · 1480 bp

Back to Lycoris

Pi 0.00437

MarkerSeek score 36.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1480
variable_sites: 21
pi: 0.00437
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGCTCCTGGAAAGTAGTCAC	CCGGGAAGGACTGATTTGAG	55.0	55.0	58.0	58.0	2537-2605 bp	1.0	81.6
atpF_p2	CGCTCCTGGAAAGTAGTCAC	CGGGAAGGACTGATTTGAGG	55.0	55.0	58.0	58.0	2536-2604 bp	1.0	81.6
atpF_p3	CGCTCCTGGAAAGTAGTCAC	CCTTCCCGTCCTTCGTTTAG	55.0	55.0	58.0	58.0	2502-2553 bp	0.904762	77.1
atpF_p4	CCGAGATTCAGAAGCGGAAA	CCTTCCCGTCCTTCGTTTAG	50.0	55.0	58.0	58.0	2239-2290 bp	0.904762	77.1
atpF_p5	AAATAAGCCTCGCTCACTGG	CCTTCCCGTCCTTCGTTTAG	50.0	55.0	58.0	58.0	2169-2220 bp	0.904762	76.9