Hotspot

rps15-ycf1

SSC · 126810-127200 · 391 bp

Back to Lycoris

Pi 0.00996

MarkerSeek score 49.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 391
variable_sites: 14
pi: 0.00996
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGAAATTCAACAGATCCGCAG	CGGCAGTCGTTTCAGTATGT	42.9	50.0	56.9	58.3	429-544 bp	1.0	67.1
rps15-ycf1_p2	GAAATTCAACAGATCCGCAGT	CGGCAGTCGTTTCAGTATGT	42.9	50.0	56.9	58.3	428-543 bp	1.0	67.1
rps15-ycf1_p3	TCAACAGATCCGCAGTTTTT	CGGCAGTCGTTTCAGTATGT	40.0	50.0	55.9	58.3	423-538 bp	1.0	67.1
rps15-ycf1_p4	TTCAACAGATCCGCAGTTTT	CGGCAGTCGTTTCAGTATGT	40.0	50.0	55.9	58.3	424-539 bp	1.0	67.1
rps15-ycf1_p5	TGAAATTCAACAGATCCGCA	CGGCAGTCGTTTCAGTATGT	40.0	50.0	55.7	58.3	429-544 bp	1.0	65.3