Hotspot

psaC-ndhE

SSC · 120559-120996 · 438 bp

Back to Lycoris

Pi 0.0122

MarkerSeek score 50.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 438
variable_sites: 12
pi: 0.0122
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	GGAAGCAGCTATTGGGCTAG	50.0	55.0	58.1	58.1	498-644 bp	1.0	84.0
psaC-ndhE_p2	CAATACATCTGTGGGGCAGG	GGAAGCAGCTATTGGGCTAG	55.0	55.0	58.3	58.1	474-620 bp	1.0	82.4
psaC-ndhE_p3	CCAATACATCTGTGGGGCAG	GGAAGCAGCTATTGGGCTAG	55.0	55.0	58.3	58.1	475-621 bp	1.0	82.4
psaC-ndhE_p4	CAGGCTCGTACACATTGAGT	GGAAGCAGCTATTGGGCTAG	50.0	55.0	57.6	58.1	458-604 bp	1.0	82.1
psaC-ndhE_p5	AGCTTTACATCCGTCCCAAG	GGAAGCAGCTATTGGGCTAG	50.0	55.0	57.6	58.1	504-650 bp	1.0	81.8