Hotspot

petA-psbJ

LSC · 65612-66770 · 1159 bp

Back to Lycoris

Pi 0.00606

MarkerSeek score 45.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1159
variable_sites: 23
pi: 0.00606
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.8	58.0	1284-1312 bp	1.0	80.0
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.8	58.1	1285-1313 bp	1.0	79.4
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.7	58.0	1291-1319 bp	1.0	75.8
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.7	58.1	1292-1320 bp	1.0	75.2
petA-psbJ_p5	GATCCATTACGCGTCCAAGG	CCGGATTGGGTTCATCTCTG	55.0	55.0	58.8	58.0	1302-1330 bp	1.0	75.0