Hotspot

ycf3

LSC · 41944-43889 · 1946 bp

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Pi 0.00308
MarkerSeek score 57.20
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
1946
variable_sites
9
pi
0.00308
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 AAAAGGCGCCTTCTTTTCTT TCGAAAATGGATTGCTGGCT 40.0 45.0 56.4 58.2 1990-1991 bp 1.0 66.5
ycf3_p2 AAAGGCGCCTTCTTTTCTTT TCGAAAATGGATTGCTGGCT 40.0 45.0 56.4 58.2 1989-1990 bp 1.0 66.5
ycf3_p3 AAGGCGCCTTCTTTTCTTTT TCGAAAATGGATTGCTGGCT 40.0 45.0 56.4 58.2 1988-1989 bp 1.0 66.5
ycf3_p4 AGGCGCCTTCTTTTCTTTTT TCGAAAATGGATTGCTGGCT 40.0 45.0 56.4 58.2 1987-1988 bp 1.0 66.5
ycf3_p5 AAGGCGCCTTCTTTTCTTTTT TCGAAAATGGATTGCTGGCT 38.1 45.0 57.2 58.2 1988-1989 bp 1.0 64.5