Hotspot

ycf1

SSC · 121965-127517 · 5553 bp

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Pi 0.00276

MarkerSeek score 54.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5553
variable_sites: 23
pi: 0.00276
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACACGTTTGCCCTTTCTTGA	ACCATTAGCAGCGGAAATTG	45.0	45.0	58.2	56.8	339 bp	1.0	68.9
ycf1_p2	ACACGTTTGCCCTTTCTTGA	CCATTAGCAGCGGAAATTGT	45.0	45.0	58.2	56.8	338 bp	1.0	68.9
ycf1_p3	CGTTTGCCCTTTCTTGAACG	ACCATTAGCAGCGGAAATTG	50.0	45.0	58.2	56.8	336 bp	1.0	68.9
ycf1_p4	CGTTTGCCCTTTCTTGAACG	CCATTAGCAGCGGAAATTGT	50.0	45.0	58.2	56.8	335 bp	1.0	68.9
ycf1_p5	ACACGTTTGCCCTTTCTTGA	CCATTAGCAGCGGAAATTGTT	45.0	42.9	58.2	57.5	338 bp	1.0	66.7