Hotspot

rpoC1

LSC · 20519-23318 · 2800 bp

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Pi 0.000952

MarkerSeek score 57.00

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2800
variable_sites: 4
pi: 0.000952
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AGTGAAGAGGTCTGCATTCC	CTGCTTGTTCGAGAACTACGA	50.0	47.6	57.2	58.1	2963-2972 bp	1.0	65.6
rpoC1_p2	GTGAAGAGGTCTGCATTCCT	CTGCTTGTTCGAGAACTACGA	50.0	47.6	57.2	58.1	2962-2971 bp	1.0	65.6
rpoC1_p3	AGTGAAGAGGTCTGCATTCC	TGCTTGTTCGAGAACTACGA	50.0	45.0	57.2	56.9	2962-2971 bp	1.0	65.6
rpoC1_p4	GTGAAGAGGTCTGCATTCCT	TGCTTGTTCGAGAACTACGA	50.0	45.0	57.2	56.9	2961-2970 bp	1.0	65.6
rpoC1_p5	GTGAAGAGGTCTGCATTCCT	ACTGCTTGTTCGAGAACTACG	50.0	47.6	57.2	58.3	2963-2972 bp	1.0	63.4