Hotspot

psbC

LSC · 33197-34618 · 1422 bp

Back to Lycopus

Pi 0.000938

MarkerSeek score 59.30

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 2
pi: 0.000938
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	CTCAAGAAATTCGTGCAGCG	ACGATGAAATCAAAGTGATTCCT	50.0	34.8	58.1	56.5	1587 bp	1.0	43.2
psbC_p2	CTCAAGAAATTCGTGCAGCG	GTGATTCCTACTTCAATTCAAGCA	50.0	37.5	58.1	57.5	1573 bp	1.0	43.1
psbC_p3	AGGTATTCGTGCTTGGATGG	ACGATGAAATCAAAGTGATTCCT	50.0	34.8	57.7	56.5	1517 bp	1.0	41.6
psbC_p4	AGGTATTCGTGCTTGGATGG	GTGATTCCTACTTCAATTCAAGCA	50.0	37.5	57.7	57.5	1503 bp	1.0	41.4
psbC_p5	CTCAAGAAATTCGTGCAGCG	AGTGATTCCTACTTCAATTCAAGC	50.0	37.5	58.1	57.3	1574 bp	1.0	41.1