Hotspot

ndhD

SSC · 113732-115261 · 1530 bp

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Pi 0.000871

MarkerSeek score 33.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 2
pi: 0.000871
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGCTCTCGCTAGCTCAAATT	CACTCATTGATTCGTCCGGT	50.0	50.0	58.1	58.0	2095-2097 bp	1.0	79.7
ndhD_p2	GGCTCTCGCTAGCTCAAATT	GGCAAGGTATGATCGTGGTT	50.0	50.0	58.1	58.0	2186-2188 bp	1.0	79.5
ndhD_p3	CTAACGAAGCATGGGGATCC	CACTCATTGATTCGTCCGGT	55.0	50.0	58.1	58.0	2446-2448 bp	1.0	79.2
ndhD_p4	CTAACGAAGCATGGGGATCC	GGCAAGGTATGATCGTGGTT	55.0	50.0	58.1	58.0	2537-2539 bp	1.0	79.0
ndhD_p5	GGCTCTCGCTAGCTCAAATT	CTGCGTTGGTTGTAAGAGGT	50.0	50.0	58.1	58.1	1861-1863 bp	1.0	78.9