Hotspot

ndhG-ndhI

SSC · 116906-117279 · 374 bp

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Pi 0.00713

MarkerSeek score 42.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 374
variable_sites: 4
pi: 0.00713
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAATGAAAAGGCCGAAT	TCAAATTGCTTTAGGCCGGT	45.0	45.0	57.8	58.1	594-596 bp	1.0	78.0
ndhG-ndhI_p2	TCCCAATGAAAAGGCCGAAT	ATTGCTTTAGGCCGGTTACC	45.0	50.0	57.8	58.2	590-592 bp	1.0	76.8
ndhG-ndhI_p3	TCCCAATGAAAAGGCCGAAT	CAAATTGCTTTAGGCCGGTT	45.0	45.0	57.8	57.3	593-595 bp	1.0	72.8
ndhG-ndhI_p4	TCCCAATGAAAAGGCCGAATA	TCAAATTGCTTTAGGCCGGT	42.9	45.0	57.6	58.1	594-596 bp	1.0	68.9
ndhG-ndhI_p5	TCCCAATGAAAAGGCCGAAT	TTGCTTTAGGCCGGTTACC	45.0	52.6	57.8	57.7	589-591 bp	1.0	68.7