Hotspot

rpl32-trnL-UAG

SSC · 111573-112329 · 757 bp

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Pi 0.00661
MarkerSeek score 55.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
757
variable_sites
7
pi
0.00661
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CTATTGGGCGGCGTTAAAAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.5 57.9 896-917 bp 1.0 75.9
rpl32-trnL-UAG_p2 AAAGGAAAGGCTATTGGGCG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.5 57.9 906-927 bp 1.0 75.4
rpl32-trnL-UAG_p3 CTATTGGGCGGCGTTAAAAG AGCAGCGTGTCTACCAATTT 50.0 45.0 57.5 57.8 889-910 bp 1.0 75.4
rpl32-trnL-UAG_p4 CTATTGGGCGGCGTTAAAAG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.5 57.8 895-916 bp 1.0 75.3
rpl32-trnL-UAG_p5 CTATTGGGCGGCGTTAAAAG CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.5 57.8 894-915 bp 1.0 75.3