Hotspot

ndhC-trnM-CAU

LSC · 49915-51701 · 1787 bp

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Pi 0.00187

MarkerSeek score 59.50

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1787
variable_sites: 5
pi: 0.00187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	CGGGGCTAAAATCCCAGAAA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.9	58.4	1928-1930 bp	1.0	75.9
ndhC-trnM-CAU_p2	AATCGGGGCTAAAATCCCAG	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.6	58.4	1931-1933 bp	1.0	74.1
ndhC-trnM-CAU_p3	CGGGGCTAAAATCCCAGAAA	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.9	57.3	1927-1929 bp	1.0	73.9
ndhC-trnM-CAU_p4	TTCTCCGGCCCTTTCCTAAT	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.4	58.4	1948-1950 bp	1.0	73.7
ndhC-trnM-CAU_p5	CGGGGCTAAAATCCCAGAAA	TTTGAACCAATGACTCCCGC	50.0	50.0	57.9	58.8	1943-1945 bp	1.0	73.1