Hotspot

rpoB-trnC-GCA

LSC · 26558-27739 · 1182 bp

Back to Lycopus

Pi 0.00113

MarkerSeek score 59.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1182
variable_sites: 2
pi: 0.00113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGTTCTTCTGCCAAACCCTG	TTTTGGTGATTCAGGCGACA	50.0	45.0	58.0	58.0	1363-1369 bp	1.0	80.2
rpoB-trnC-GCA_p2	GTTCTTCTGCCAAACCCTGA	TTTTGGTGATTCAGGCGACA	50.0	45.0	58.0	58.0	1362-1368 bp	1.0	80.1
rpoB-trnC-GCA_p3	AGTTCTTCTGCCAAACCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1342-1348 bp	1.0	79.7
rpoB-trnC-GCA_p4	GTTCTTCTGCCAAACCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1341-1347 bp	1.0	79.7
rpoB-trnC-GCA_p5	GCCAAACCCTGATCGATGAA	TTTTGGTGATTCAGGCGACA	50.0	45.0	58.2	58.0	1354-1360 bp	1.0	78.3