Hotspot

trnK-UUU-rps16

LSC · 4297-4851 · 555 bp

Back to Lycopus

Pi 0.00781

MarkerSeek score 62.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 555
variable_sites: 6
pi: 0.00781
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTCAAAAGTT	CTTTTAAAGAAGGCGGGGGT	45.0	50.0	58.1	58.1	731-732 bp	1.0	79.7
trnK-UUU-rps16_p2	AGTCGCGGTCTTCAAAAGTT	TCTTTTAAAGAAGGCGGGGG	45.0	50.0	58.1	57.8	732-733 bp	1.0	78.7
trnK-UUU-rps16_p3	TCTAGCCGCACTTAAAAGCC	CTTTTAAAGAAGGCGGGGGT	50.0	50.0	58.3	58.1	662-663 bp	1.0	78.1
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	CTTTTAAAGAAGGCGGGGGT	50.0	50.0	58.3	58.1	657-658 bp	1.0	77.9
trnK-UUU-rps16_p5	AGTCGCGGTCTTCAAAAGTT	AGGCGGGGGTTTTTAAAGAA	45.0	45.0	58.1	57.6	721-722 bp	1.0	77.3