Hotspot

ndhD

Genome · 132531-134039 · 1509 bp

Back to Lycopodium

Pi 0.00265

MarkerSeek score 47.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: Genome
length_bp: 1509
variable_sites: 4
pi: 0.00265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAAGGTGTGAATCCGCTTGT	TCCCTCAGGAAAAGGTTTGC	45.0	50.0	58.0	58.0	2016-2017 bp	1.0	80.4
ndhD_p2	TATTGGTTGTACCCAGTGCG	TCCCTCAGGAAAAGGTTTGC	50.0	50.0	57.9	58.0	2138-2139 bp	1.0	79.7
ndhD_p3	AAAGGTGTGAATCCGCTTGT	TCTCCTATGCGGATCCCTTT	45.0	50.0	58.0	57.9	2484-2485 bp	1.0	79.5
ndhD_p4	TGATACCTTGGGACGGATGT	TCCCTCAGGAAAAGGTTTGC	50.0	50.0	58.1	58.0	2088-2089 bp	1.0	79.5
ndhD_p5	TGGGACGGATGTAAGGCTAA	TCCCTCAGGAAAAGGTTTGC	50.0	50.0	58.1	58.0	2080-2081 bp	1.0	79.5