Hotspot

ndhD-ccsA

Genome · 134040-134263 · 224 bp

Back to Lycopodium

Pi 0.0359
MarkerSeek score 70.50
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
Genome
length_bp
224
variable_sites
8
pi
0.0359
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 TCCTAACCTGGTTTTATCTCT TCCCTCAGGAAAAGGTTTGC 38.1 50.0 53.3 58.0 342-343 bp 1.0 42.1
ndhD-ccsA_p2 TCCTAACCTGGTTTTATCTCT CCCTCAGGAAAAGGTTTGCA 38.1 50.0 53.3 58.3 341-342 bp 1.0 42.1
ndhD-ccsA_p3 ATCCTAACCTGGTTTTATCTCT TCCCTCAGGAAAAGGTTTGC 36.4 50.0 53.9 58.0 343-344 bp 1.0 42.1
ndhD-ccsA_p4 AGGCATAGGTTTATATCCTAACC TCCCTCAGGAAAAGGTTTGC 39.1 50.0 55.0 58.0 357-358 bp 1.0 42.0
ndhD-ccsA_p5 AGGCATAGGTTTATATCCTAACC CCCTCAGGAAAAGGTTTGCA 39.1 50.0 55.0 58.3 356-357 bp 1.0 42.0