Hotspot

trnL-CAA-ndhB

Genome · 100652-101699 · 1048 bp

Back to Lycopodium

Pi 0.0144

MarkerSeek score 66.50

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: Genome
length_bp: 1048
variable_sites: 15
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	CTCGCGTGTCTACCATTTCA	TGCGTAGTAGCATCTACCGT	50.0	50.0	58.0	58.3	1165-1171 bp	1.0	78.9
trnL-CAA-ndhB_p2	CAATGCCCTGAAGAGGACTC	TGCGTAGTAGCATCTACCGT	55.0	50.0	58.0	58.3	1219-1225 bp	1.0	78.6
trnL-CAA-ndhB_p3	GCTTTGCAGCACGAGATTTT	TGCGTAGTAGCATCTACCGT	45.0	50.0	57.9	58.3	1189-1195 bp	1.0	78.5
trnL-CAA-ndhB_p4	TTTCTGGAGTTCCAATGCCC	TGCGTAGTAGCATCTACCGT	50.0	50.0	58.1	58.3	1231-1237 bp	1.0	78.3
trnL-CAA-ndhB_p5	GACTCGAACCTCCATGCTTT	TGCGTAGTAGCATCTACCGT	50.0	50.0	57.9	58.3	1204-1210 bp	1.0	78.1