Hotspot

trnG-UCC-trnR-UCU

Genome · 78974-80455 · 1482 bp

Back to Lycopodium

Pi 0.0108

MarkerSeek score 58.60

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: Genome
length_bp: 1482
variable_sites: 16
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	ACCCTTAGCCTTCCAAGCTA	GGATTTGAACCTATACCGGAAGT	50.0	43.5	58.0	58.0	1593-1861 bp	1.0	56.3
trnG-UCC-trnR-UCU_p2	ACCCTTAGCCTTCCAAGCTA	CTGTCCTATCCATTAGACGATGG	50.0	47.8	58.0	57.9	1559-1827 bp	1.0	56.1
trnG-UCC-trnR-UCU_p3	ACCCTTAGCCTTCCAAGCTA	TGTCCTATCCATTAGACGATGG	50.0	45.5	58.0	56.9	1558-1826 bp	1.0	55.5
trnG-UCC-trnR-UCU_p4	ACCCTTAGCCTTCCAAGCTA	AGGATTTGAACCTATACCGGA	50.0	42.9	58.0	55.8	1594-1862 bp	1.0	55.3
trnG-UCC-trnR-UCU_p5	ACCCTTAGCCTTCCAAGCTA	AGGATTTGAACCTATACCGGAAG	50.0	43.5	58.0	57.7	1594-1862 bp	1.0	54.2