Hotspot

trnfM-CAU-trnG-GCC

Genome · 53601-53727 · 127 bp

Back to Lycopodium

Pi 0.0236

MarkerSeek score 66.10

Primer available yes

Label trnfM-CAU-trnG-GCC

Feature Metadata

label_name: trnfM-CAU-trnG-GCC
feature_type: igs
region: Genome
length_bp: 127
variable_sites: 3
pi: 0.0236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnG-GCC_p1	CTTGAGGTCACGGGTTCAAA	AGAAGATGCGGGTTCGATTC	50.0	50.0	58.0	58.1	193 bp	1.0	80.5
trnfM-CAU-trnG-GCC_p2	CTTGAGGTCACGGGTTCAAA	GAGAAGATGCGGGTTCGATT	50.0	50.0	58.0	58.1	194 bp	1.0	80.5
trnfM-CAU-trnG-GCC_p3	ATGAATTGCGCGGAGTAGAG	AGAAGATGCGGGTTCGATTC	50.0	50.0	58.1	58.1	241 bp	1.0	79.4
trnfM-CAU-trnG-GCC_p4	ATGAATTGCGCGGAGTAGAG	GAGAAGATGCGGGTTCGATT	50.0	50.0	58.1	58.1	242 bp	1.0	79.4
trnfM-CAU-trnG-GCC_p5	TGAGGTCACGGGTTCAAATC	AGAAGATGCGGGTTCGATTC	50.0	50.0	57.8	58.1	191 bp	1.0	79.4