Hotspot

trnS-GGA-ycf3

Genome · 45221-45823 · 603 bp

Back to Lycopodium

Pi 0.0103

MarkerSeek score 63.30

Primer available yes

Label trnS-GGA-ycf3

Feature Metadata

label_name: trnS-GGA-ycf3
feature_type: igs
region: Genome
length_bp: 603
variable_sites: 6
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-ycf3_p1	GAACCCTCGGTAAGCAAGAG	TTCCCTTTGAGTAGTCGGAA	55.0	45.0	58.0	55.8	754-769 bp	1.0	65.1
trnS-GGA-ycf3_p2	GAACCCTCGGTAAGCAAGAG	TTTCCCTTTGAGTAGTCGGA	55.0	45.0	58.0	55.8	755-770 bp	1.0	65.0
trnS-GGA-ycf3_p3	TGCTACGCCTTAAACCACTC	TTCCCTTTGAGTAGTCGGAA	50.0	45.0	57.9	55.8	716-731 bp	1.0	64.6
trnS-GGA-ycf3_p4	TGCTACGCCTTAAACCACTC	TTTCCCTTTGAGTAGTCGGA	50.0	45.0	57.9	55.8	717-732 bp	1.0	64.6
trnS-GGA-ycf3_p5	CTCGGTAAGCAAGAGCCTAC	TTCCCTTTGAGTAGTCGGAA	55.0	45.0	57.8	55.8	749-764 bp	1.0	63.5