Hotspot

rps4-trnS-GGA

Genome · 44769-45133 · 365 bp

Back to Lycopodium

Pi 0.0165
MarkerSeek score 63.60
Primer available yes
Label rps4-trnS-GGA

Feature Metadata

label_name
rps4-trnS-GGA
feature_type
igs
region
Genome
length_bp
365
variable_sites
6
pi
0.0165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnS-GGA_p1 AACGAATTGCTAGTTGTGGA CTCTTGCTTACCGAGGGTTC 40.0 55.0 55.3 58.0 442-444 bp 1.0 59.7
rps4-trnS-GGA_p2 ACGAATTGCTAGTTGTGGAA CTCTTGCTTACCGAGGGTTC 40.0 55.0 55.3 58.0 441-443 bp 1.0 59.7
rps4-trnS-GGA_p3 AACGAATTGCTAGTTGTGGAA CTCTTGCTTACCGAGGGTTC 38.1 55.0 56.1 58.0 442-444 bp 1.0 58.1
rps4-trnS-GGA_p4 AACGAATTGCTAGTTGTGGA GTAGGCTCTTGCTTACCGAG 40.0 55.0 55.3 57.8 447-449 bp 1.0 58.1
rps4-trnS-GGA_p5 ACGAATTGCTAGTTGTGGAA GTAGGCTCTTGCTTACCGAG 40.0 55.0 55.3 57.8 446-448 bp 1.0 58.1