Hotspot

trnV-UAC-ndhC

Genome · 38821-38975 · 155 bp

Back to Lycopodium

Pi 0.0194

MarkerSeek score 63.60

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: Genome
length_bp: 155
variable_sites: 3
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	TGATCGAACCGTTTAACCGA	TGGGGGAATAAGACCAGCTA	45.0	50.0	57.3	57.4	273 bp	1.0	70.5
trnV-UAC-ndhC_p2	TCGAACCGTTTAACCGAGAA	TGGGGGAATAAGACCAGCTA	45.0	50.0	57.2	57.4	270 bp	1.0	69.9
trnV-UAC-ndhC_p3	TGATCGAACCGTTTAACCGA	ACAGGTGCTAAAAAGCTGGA	45.0	45.0	57.3	57.3	308 bp	1.0	69.8
trnV-UAC-ndhC_p4	TCGAACCGTTTAACCGAGAA	ACAGGTGCTAAAAAGCTGGA	45.0	45.0	57.2	57.3	305 bp	1.0	69.2
trnV-UAC-ndhC_p5	TGATCGAACCGTTTAACCGA	CTGGGGGAATAAGACCAGCT	45.0	55.0	57.3	58.8	274 bp	1.0	68.8