Hotspot

rbcL-atpB

Genome · 35311-35934 · 624 bp

Back to Lycopodium

Pi 0.00647

MarkerSeek score 63.40

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: Genome
length_bp: 624
variable_sites: 4
pi: 0.00647
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	ATGCTGCCAGAATATCGGTG	TGGGCATCTTACCTGGAGAA	50.0	50.0	58.1	58.0	854-860 bp	1.0	79.8
rbcL-atpB_p2	ATGCTGCCAGAATATCGGTG	TTGGGCATCTTACCTGGAGA	50.0	50.0	58.1	58.0	855-861 bp	1.0	79.8
rbcL-atpB_p3	GTCTCCGTTTGTGGTGACAT	TGGGCATCTTACCTGGAGAA	50.0	50.0	58.1	58.0	756-762 bp	1.0	79.8
rbcL-atpB_p4	GTCTCCGTTTGTGGTGACAT	TTGGGCATCTTACCTGGAGA	50.0	50.0	58.1	58.0	757-763 bp	1.0	79.8
rbcL-atpB_p5	ATGCTGCCAGAATATCGGTG	ACATCTAGGACTGGGCCAAT	50.0	50.0	58.1	58.1	828-834 bp	1.0	79.3