Hotspot

trnR-CCG-rbcL

Genome · 33676-33882 · 207 bp

Back to Lycopodium

Pi 0.039
MarkerSeek score 75.80
Primer available yes
Label trnR-CCG-rbcL

Feature Metadata

label_name
trnR-CCG-rbcL
feature_type
igs
region
Genome
length_bp
207
variable_sites
8
pi
0.039
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-CCG-rbcL_p1 GGGTCAGAAGGGATTCGAAC GCTTGTAAGTGGAGTGCTGA 55.0 50.0 58.0 57.8 348-367 bp 1.0 84.2
trnR-CCG-rbcL_p2 GGTCAGAAGGGATTCGAACC GCTTGTAAGTGGAGTGCTGA 55.0 50.0 58.0 57.8 347-366 bp 1.0 84.2
trnR-CCG-rbcL_p3 GGGTCAGAAGGGATTCGAAC AGCTTGTAAGTGGAGTGCTG 55.0 50.0 58.0 57.8 349-368 bp 1.0 84.2
trnR-CCG-rbcL_p4 GGTCAGAAGGGATTCGAACC AGCTTGTAAGTGGAGTGCTG 55.0 50.0 58.0 57.8 348-367 bp 1.0 84.2
trnR-CCG-rbcL_p5 ATGGGTCAGAAGGGATTCGA GCTTGTAAGTGGAGTGCTGA 50.0 50.0 58.1 57.8 350-369 bp 1.0 83.5