Hotspot

ycf1

SSC · 125763-131438 · 5676 bp

Back to Lycium

Pi 0.00364
MarkerSeek score 40.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5676
variable_sites
137
pi
0.00364
unique_haplotype_count
40
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ACGGCCTGTTCAAATCGATT CGGACAAAATCGACGAAACG 45.0 50.0 58.2 58.2 313-319 bp 1.0 84.1
ycf1_p2 ACGGCCTGTTCAAATCGATT CCGGACAAAATCGACGAAAC 45.0 50.0 58.2 57.8 314-320 bp 1.0 83.6
ycf1_p3 ACGGCCTGTTCAAATCGATT GGACAAAATCGACGAAACGG 45.0 50.0 58.2 57.8 312-318 bp 1.0 83.6
ycf1_p4 ACGGCCTGTTCAAATCGATT GATCCGGACAAAATCGACGA 45.0 50.0 58.2 58.1 323 bp 0.057692 40.5
ycf1_p5 ACGGCCTGTTCAAATCGATT TCCGGACAAAATCGACGAAA 45.0 45.0 58.2 57.8 321 bp 0.057692 39.9