Hotspot

rpl32-trnL-UAG

SSC · 115276-116038 · 763 bp

Back to Lycium

Pi 0.00807
MarkerSeek score 55.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
763
variable_sites
27
pi
0.00807
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCGAAATCTCTTTCTACCGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.8 57.9 869-899 bp 1.0 73.8
rpl32-trnL-UAG_p2 AGCGAAATCTCTTTCTACCGG TCGAACCGAGATGCATTAGC 47.6 50.0 57.8 58.1 895-925 bp 1.0 73.5
rpl32-trnL-UAG_p3 AGCGAAATCTCTTTCTACCGG AGCAGCGTGTCTACCAATTT 47.6 45.0 57.8 57.8 862-892 bp 1.0 73.4
rpl32-trnL-UAG_p4 AGCGAAATCTCTTTCTACCGG CCTAAGAGCAGCGTGTCTAC 47.6 55.0 57.8 57.8 868-898 bp 1.0 73.3
rpl32-trnL-UAG_p5 AGCGAAATCTCTTTCTACCGG CTAAGAGCAGCGTGTCTACC 47.6 55.0 57.8 57.8 867-897 bp 1.0 73.3