Hotspot

ycf4-cemA

LSC · 62647-63373 · 727 bp

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Pi 0.00566
MarkerSeek score 39.70
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
727
variable_sites
25
pi
0.00566
unique_haplotype_count
24
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 CATTCCCTTGACCCGTACTG GGAATTCCTGAGCCCAAGAG 55.0 55.0 58.0 58.0 1249-1257 bp 1.0 82.0
ycf4-cemA_p2 CGGAATTGCGGGTCTCTTTA GAAAGAAATCCACCAGGGCA 50.0 50.0 58.0 58.1 1146-1154 bp 1.0 81.8
ycf4-cemA_p3 TCTTTCTACGGAATTGCGGG GAAAGAAATCCACCAGGGCA 50.0 50.0 58.0 58.1 1154-1162 bp 1.0 81.8
ycf4-cemA_p4 CGGAATTGCGGGTCTCTTTA GGAATTCCTGAGCCCAAGAG 50.0 55.0 58.0 58.0 1495-1503 bp 1.0 81.7
ycf4-cemA_p5 TCTTTCTACGGAATTGCGGG GGAATTCCTGAGCCCAAGAG 50.0 55.0 58.0 58.0 1503-1511 bp 1.0 81.7