Hotspot

rpoB-trnC-GCA

LSC · 27095-28399 · 1305 bp

Back to Lycium

Pi 0.00328
MarkerSeek score 55.10
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1305
variable_sites
28
pi
0.00328
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CTCATTTCCATCCCCGAGC CCGGATTTGAACTGGGGAAA 57.9 50.0 58.0 58.1 1374-1405 bp 1.0 75.6
rpoB-trnC-GCA_p2 ATTCCCTCATTTCCATCCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 56.9 58.1 1379-1410 bp 1.0 75.0
rpoB-trnC-GCA_p3 TCATTTCCATCCCCGAGCAT CCGGATTTGAACTGGGGAAA 50.0 50.0 59.2 58.1 1373-1404 bp 1.0 74.4
rpoB-trnC-GCA_p4 CTCATTTCCATCCCCGAGC AGTCCTCTGCCTTACCACTC 57.9 55.0 58.0 58.4 1343-1374 bp 1.0 72.8
rpoB-trnC-GCA_p5 CATTTCCATCCCCGAGCAT CCGGATTTGAACTGGGGAAA 52.6 50.0 57.6 58.1 1372-1403 bp 1.0 72.3