Hotspot

rpl16

LSC · 125230-126721 · 1492 bp

Back to Lutheria

Pi 0.00614

MarkerSeek score 50.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1492
variable_sites: 9
pi: 0.00614
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	GGTTTCGAAGGAATTGCACG	50.0	50.0	58.0	58.0	2553-2565 bp	1.0	80.9
rpl16_p2	ATCAATTTTCGAGCTCCGCT	GGTTTCGAAGGAATTGCACG	45.0	50.0	58.0	58.0	2309-2321 bp	1.0	80.9
rpl16_p3	TTTCGAGCTCCGCTGTTATC	GGTTTCGAAGGAATTGCACG	50.0	50.0	58.1	58.0	2303-2315 bp	1.0	80.5
rpl16_p4	CATCAATTTTCGAGCTCCGC	GGTTTCGAAGGAATTGCACG	50.0	50.0	57.9	58.0	2310-2322 bp	1.0	80.4
rpl16_p5	ATCGCACCAAAAACTCGAGT	GGTTTCGAAGGAATTGCACG	45.0	50.0	58.1	58.0	2555-2567 bp	1.0	80.1