Hotspot

ndhF

SSC · 154723-156936 · 2214 bp

Back to Lutheria

Pi NA

MarkerSeek score 31.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 31.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	ACTCCCCTCCCAAAATGAAC	50.0	50.0	58.0	57.4	2874 bp	0.5	54.8
ndhF_p2	AAGAGGGATCCACCGAAGAA	ACTCCCCTCCCAAAATGAAC	50.0	50.0	58.0	57.4	2866 bp	0.5	54.8
ndhF_p3	AGCCCAGTTCACGAAGATTC	ACTCCCCTCCCAAAATGAAC	50.0	50.0	57.9	57.4	2714 bp	0.5	54.4
ndhF_p4	GCCCAGTTCACGAAGATTCT	ACTCCCCTCCCAAAATGAAC	50.0	50.0	57.9	57.4	2713 bp	0.5	54.4
ndhF_p5	GCCCAGTTCACGAAGATTCT	CACATATACGTATGCGCGTG	50.0	50.0	57.9	57.3	2927 bp	0.5	53.2