Hotspot

cemA

LSC · 104419-105108 · 690 bp

Back to Lutheria

Pi 0.0058

MarkerSeek score 49.30

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 4
pi: 0.0058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TCATTAACGATTCAATTCACAGA	TCAGGAGATCAAATGAGTTCT	30.4	38.1	54.4	54.0	740 bp	1.0	40.4
cemA_p2	TTCATTAACGATTCAATTCACAGA	TCAGGAGATCAAATGAGTTCT	29.2	38.1	55.1	54.0	741 bp	1.0	40.4
cemA_p3	TCATTAACGATTCAATTCACAGA	ATCAGGAGATCAAATGAGTTCT	30.4	36.4	54.4	54.6	741 bp	1.0	40.4
cemA_p4	TCATTAACGATTCAATTCACAGAT	TCAGGAGATCAAATGAGTTCT	29.2	38.1	54.9	54.0	740 bp	1.0	40.4
cemA_p5	CATTAACGATTCAATTCACAGA	TCAGGAGATCAAATGAGTTCT	31.8	38.1	52.9	54.0	739 bp	1.0	40.4