Hotspot

psbT-psbN

LSC · 118246-118306 · 61 bp

Back to Lutheria

Pi 0.0164

MarkerSeek score 61.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 1
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTCGAGAACCGCCTAAGG	ATCCATTCGAAGAACACGGG	50.0	50.0	57.9	58.0	130 bp	1.0	79.7
psbT-psbN_p2	TTTTCGAGAACCGCCTAAGG	TCGCCATCTCCATATCTGGT	50.0	50.0	57.9	58.0	217 bp	1.0	79.5
psbT-psbN_p3	ACGATCGAATCCATGGAAGC	ATCCATTCGAAGAACACGGG	50.0	50.0	58.1	58.0	207 bp	1.0	79.2
psbT-psbN_p4	ACGATCGAATCCATGGAAGC	TCGCCATCTCCATATCTGGT	50.0	50.0	58.1	58.0	294 bp	1.0	79.1
psbT-psbN_p5	CGATCGAATCCATGGAAGCA	TCGCCATCTCCATATCTGGT	50.0	50.0	58.1	58.0	293 bp	1.0	79.1