Hotspot

ndhJ-ndhK

LSC · 92228-92302 · 75 bp

Back to Lutheria

Pi 0.0267

MarkerSeek score 67.80

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 75
variable_sites: 2
pi: 0.0267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	CGGTAATCGAAGCCCAAAGA	GTGAATCAGGCAGGGTTCTT	50.0	50.0	58.0	57.8	182-197 bp	1.0	79.0
ndhJ-ndhK_p2	TCGGTAATCGAAGCCCAAAG	GTGAATCAGGCAGGGTTCTT	50.0	50.0	58.0	57.8	183-198 bp	1.0	79.0
ndhJ-ndhK_p3	CGGTAATCGAAGCCCAAAGA	TCAGGCAGGGTTCTTTTGTG	50.0	50.0	58.0	58.3	177-192 bp	1.0	78.2
ndhJ-ndhK_p4	TCGGTAATCGAAGCCCAAAG	TCAGGCAGGGTTCTTTTGTG	50.0	50.0	58.0	58.3	178-193 bp	1.0	78.2
ndhJ-ndhK_p5	CGGTAATCGAAGCCCAAAGA	AGTGAATCAGGCAGGGTTCT	50.0	50.0	58.0	58.6	183-198 bp	1.0	75.6