Hotspot

trnK-UUU-rps16

LSC · 46028-46719 · 692 bp

Back to Lutheria

Pi 0.0131

MarkerSeek score 62.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 9
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	796-801 bp	1.0	78.5
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	795-800 bp	1.0	78.5
trnK-UUU-rps16_p3	TACCGTTGAGTTAGCAACCC	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.5	58.2	782-787 bp	1.0	76.2
trnK-UUU-rps16_p4	AGTCGCACTTAAAAGCCGAG	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.6	58.2	807-812 bp	1.0	75.3
trnK-UUU-rps16_p5	TCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGGAAC	45.0	55.0	57.3	58.2	805-810 bp	1.0	74.0