Hotspot

ndhE-ndhG

SSC · 4863-5081 · 219 bp

Back to Lutheria

Pi 0.02

MarkerSeek score 67.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 219
variable_sites: 4
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GCTCAAGACACATAAGCGCT	AGGTGCAATTACTATGGCTCG	50.0	47.6	58.6	58.2	334-353 bp	1.0	70.6
ndhE-ndhG_p2	AGCGCTCTAACCATGTTTCG	AGGTGCAATTACTATGGCTCG	50.0	47.6	58.6	58.2	320-339 bp	1.0	70.6
ndhE-ndhG_p3	AGCTCAAGACACATAAGCGC	AGGTGCAATTACTATGGCTCG	50.0	47.6	58.6	58.2	335-354 bp	1.0	70.5
ndhE-ndhG_p4	AGCTCAAGACACATAAGCGC	GGTGCAATTACTATGGCTCGA	50.0	47.6	58.6	58.2	334-353 bp	1.0	70.5
ndhE-ndhG_p5	GCTCAAGACACATAAGCGCT	GGTGCAATTACTATGGCTCGA	50.0	47.6	58.6	58.2	333-352 bp	1.0	70.5