Hotspot

rpoC2

LSC · 24727-28875 · 4149 bp

Back to Lupinus

Pi 0.00988

MarkerSeek score 47.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4149
variable_sites: 86
pi: 0.00988
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGCCAATTCAAATGGAATGGA	GGCCCTTTAGGAATAACCGT	38.1	50.0	56.4	57.3	215 bp	1.0	63.2
rpoC2_p2	TCGGGCTTATAAATTCTCTCGT	GGCCCTTTAGGAATAACCGT	40.9	50.0	57.1	57.3	170 bp	1.0	62.8
rpoC2_p3	TGCCAATTCAAATGGAATGGA	GGCCCTTTAGGAATAACCGTT	38.1	47.6	56.4	58.0	215 bp	1.0	60.9
rpoC2_p4	TGCCAATTCAAATGGAATGGA	AGGCCCTTTAGGAATAACCG	38.1	50.0	56.4	57.0	216 bp	1.0	60.7
rpoC2_p5	TGCCAATTCAAATGGAATGGAT	GGCCCTTTAGGAATAACCGT	36.4	50.0	56.9	57.3	215 bp	1.0	59.3