Hotspot

trnN-GUU-trnR-ACG

IRa · 127420-127994 · 575 bp

Back to Lupinus

Pi 0.0153

MarkerSeek score 69.40

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 575
variable_sites: 19
pi: 0.0153
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	715-716 bp	1.0	80.5
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	752-753 bp	1.0	79.8
trnN-GUU-trnR-ACG_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	756-757 bp	1.0	79.6
trnN-GUU-trnR-ACG_p4	AGCTCGAATTTCCCGTTGTT	CAGAGGATTAGAGCACGTGG	45.0	55.0	58.1	57.8	727-728 bp	1.0	79.6
trnN-GUU-trnR-ACG_p5	CCGTTGTTCCTCAGTAGCTC	TTTTAGGGTACTCCGGGGAA	55.0	50.0	58.0	57.7	769-770 bp	1.0	79.3