Hotspot

petD-rpoA

LSC · 75992-76209 · 218 bp

Back to Lupinus

Pi 0.0321

MarkerSeek score 65.70

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 218
variable_sites: 17
pi: 0.0321
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTTGGGTATTGGAGCAACA	CGAGAAATCGCGGATATCGT	50.0	50.0	58.1	58.0	814-820 bp	1.0	82.3
petD-rpoA_p2	TGGTTGGGTATTGGAGCAAC	CGAGAAATCGCGGATATCGT	50.0	50.0	58.1	58.0	815-821 bp	1.0	82.3
petD-rpoA_p3	GTTTGATTCGTGCTTGAGCC	GAAGCCTCCCGGAATTTGAT	50.0	50.0	58.0	57.9	1124-1130 bp	1.0	82.0
petD-rpoA_p4	GTTTGATTCGTGCTTGAGCC	CGAGAAATCGCGGATATCGT	50.0	50.0	58.0	58.0	1318-1324 bp	1.0	81.8
petD-rpoA_p5	GTGCTTGAGCCGTATGATGA	CGAGAAATCGCGGATATCGT	50.0	50.0	58.1	58.0	1309-1315 bp	1.0	81.6