Hotspot

trnP-UGG-psaJ

LSC · 64647-65087 · 441 bp

Back to Lupinus

Pi 0.0228

MarkerSeek score 69.90

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 22
pi: 0.0228
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GCTAAAGCCCCGAACCATAA	50.0	50.0	58.1	58.0	544-567 bp	1.0	85.8
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GCTAAAGCCCCGAACCATAA	50.0	50.0	58.1	58.0	543-566 bp	1.0	85.8
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GCTAAAGCCCCGAACCATAA	50.0	50.0	58.1	58.0	555-578 bp	1.0	85.6
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GCTAAAGCCCCGAACCATAA	50.0	50.0	58.1	58.0	554-577 bp	1.0	85.6
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GCTAAAGCCCCGAACCATAA	50.0	50.0	58.1	58.0	557-580 bp	1.0	85.6